4U8H

Crystal Structure of Mammalian Period-Cryptochrome Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 41
Unit cell lengths	97.675, 97.675, 163.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.738 - 2.798
R-factor	0.2088
Rwork	0.205
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mlp
RMSD bond length	0.009
RMSD bond angle	1.345
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.738
High resolution limit [Å]	2.798
Number of reflections	37522
<I/σ(I)>	18.8
Completeness [%]	99.6
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M HEPES, 0.2 M NaCl, 15% PEG3350