4U7C
Structure of DNA polymerase kappa in complex with benzopyrene adducted DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-05-13 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.640, 130.170, 166.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.111 - 2.800 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.057 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.111 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.671 |
| Number of reflections | 34235 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 98.7 | 98.4 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | PEG 400, ammonium iodide |
