4U73
HsMetAP(F309M) IN COMPLEX WITH (amino(phenyl)methyl)phosphonic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-03-26 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.477, 77.296, 48.271 |
Unit cell angles | 90.00, 91.19, 90.00 |
Refinement procedure
Resolution | 15.750 - 1.800 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.20100 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.987 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 16.000 |
High resolution limit [Å] | 1.800 |
Number of reflections | 32452 |
<I/σ(I)> | 3.58 |
Completeness [%] | 99.5 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15% PEGMME, 100mM HEPES PH 6 |