4U6W
HsMetAP (F220M) in complex with 1-amino-2-propylpentyl]phosphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.149, 77.099, 47.487 |
| Unit cell angles | 90.00, 91.29, 90.00 |
Refinement procedure
| Resolution | 17.860 - 1.830 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.22400 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.979 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 33117 |
| <I/σ(I)> | 2 |
| Completeness [%] | 99.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 15% PEG-MME, 100mM Hepes, PH 6 |
