4U6C
HsMetAP in complex with [(1R)-1-amino-3-cyclopentylpropyl]phosphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.076, 77.299, 47.420 |
| Unit cell angles | 90.00, 91.38, 90.00 |
Refinement procedure
| Resolution | 21.000 - 1.910 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.22900 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.907 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.640 |
| High resolution limit [Å] | 1.870 |
| Number of reflections | 26672 |
| <I/σ(I)> | 2.94 |
| Completeness [%] | 95.5 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 2000, potassium chloride, hepes, sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
