4U5C

Crystal structure of GluA2, con-ikot-ikot snail toxin, partial agonist FW and postitive modulator (R,R)-2b complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	163.070, 364.780, 109.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.984 - 3.688
R-factor	0.2097
Rwork	0.207
R-free	0.25360
RMSD bond length	0.004
RMSD bond angle	0.883
Data scaling software	XDS
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	148.870	3.780
High resolution limit [Å]	3.688	3.688
Rmerge		0.734
Number of reflections	69997
<I/σ(I)>	11.8	1.9
Completeness [%]	98.2	95.1
Redundancy	3.7	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MES pH 5.8-6.3, 0.1 M NaCl, 5%-6% PEG3350