4U5B

Crystal structure of GluA2 A622T, con-ikot-ikot snail toxin, partial agonist KA and postitive modulator (R,R)-2b complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	161.520, 368.660, 109.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.998 - 3.504
R-factor	0.2216
Rwork	0.220
R-free	0.26060
RMSD bond length	0.003
RMSD bond angle	0.769
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	90.440
High resolution limit [Å]	3.500
Number of reflections	79285
<I/σ(I)>	7.5
Completeness [%]	96.1
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MES pH 5.8-6.3, 0.1 M NaCl, 5%-6% PEG3350