4U3Z

APO MAP4K4 T181E Phosphomimetic Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-15
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	79.720, 80.900, 92.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.407 - 2.090
R-factor	0.186
Rwork	0.183
R-free	0.24900
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.081
Data scaling software	SCALA
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.000	2.200
High resolution limit [Å]	2.090	2.090
Number of reflections	36129
<I/σ(I)>	11.2	2.7
Completeness [%]	100.0	100
Redundancy	5.3	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	292	0.2 M potassium citrate, pH 8.3, 20% PEG 3350