4U3E

Anaerobic ribonucleotide reductase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9790
Spacegroup name	P 1 21 1
Unit cell lengths	78.313, 98.768, 86.556
Unit cell angles	90.00, 111.72, 90.00

Refinement procedure

Resolution	49.380 - 1.640
R-factor	0.171
Rwork	0.171
R-free	0.20000
RMSD bond length	0.005
RMSD bond angle	0.928
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.670
High resolution limit [Å]	1.640	1.640
Rmerge		0.653
Number of reflections	279509
<I/σ(I)>	10.7	0.97
Completeness [%]	94.4	65.4
Redundancy	1.8	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	298	0.085 M tri-sodium citrate pH 5.6, 0.17 M ammonium acetate, 25.5% PEG 4000, 15% glycerol. Diffraction quality crystals grew over several weeks.