4U39

Crystal Structure of FtsZ:MciZ Complex from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0044
Spacegroup name	P 65 2 2
Unit cell lengths	167.360, 167.360, 528.640
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.593 - 3.194
R-factor	0.2349
Rwork	0.232
R-free	0.29180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vxy
RMSD bond length	0.011
RMSD bond angle	1.422
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.593	3.390
High resolution limit [Å]	3.190	3.190
Rmerge		0.370
Number of reflections	72155
<I/σ(I)>	62.64	12.7
Completeness [%]	97.5	91.2
Redundancy	17.33

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.6 M sodium/potassium phosphate, pH 7.0