4U30
Human mesotrypsin complexed with bikunin Kunitz domain 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 3 |
| Unit cell lengths | 164.000, 164.000, 81.021 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.750 - 2.500 |
| R-factor | 0.18954 |
| Rwork | 0.188 |
| R-free | 0.22284 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.928 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 80120 |
| <I/σ(I)> | 2 |
| Completeness [%] | 99.0 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1 M HEPES pH 7.0 and 1 M sodium citrate |
