4U2X

Ebola virus VP24 in complex with Karyopherin alpha 5 C-terminus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792601
Spacegroup name	P 31 2 1
Unit cell lengths	103.925, 103.925, 333.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.120 - 3.153
R-factor	0.2163
Rwork	0.215
R-free	0.24920
RMSD bond length	0.004
RMSD bond angle	0.824
Data reduction software	HKL-3000
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.200
High resolution limit [Å]	3.150	8.540	3.150
Rmerge	0.091	0.032	0.953
Total number of observations	214292
Number of reflections	35719
<I/σ(I)>	8.1
Completeness [%]	96.2	89.5	98.1
Redundancy	6	5.8	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	3.5M ammonium chloride, 0.1 M Bis-Tris propane