4U2N
Crystal structure of a complex of the Miz1- and Nac1 POZ domains.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-25 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 109.090, 115.190, 58.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.930 - 2.300 |
R-factor | 0.2402 |
Rwork | 0.238 |
R-free | 0.28630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u2m |
RMSD bond length | 0.017 |
RMSD bond angle | 1.623 |
Data reduction software | XDS (2.5.6) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.930 | 2.360 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.030 | 0.663 |
Number of reflections | 33315 | |
<I/σ(I)> | 24 | 2.4 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.2 % PEG 4000, 0.1 M sodium citrate pH 5.6 |