4U2H
The crystal structure of apo CalE6, a methionine gamma lyase from Micromonospora echinospora
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 145.204, 145.395, 346.119 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.300 - 1.850 |
Rwork | 0.209 |
R-free | 0.23160 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.300 |
High resolution limit [Å] | 1.850 |
Number of reflections | 308727 |
<I/σ(I)> | 10.3 |
Completeness [%] | 99.8 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2 M lithium sulfate, 0.1 M Bis-Tris, 25% PEG3350 |