4U2H
The crystal structure of apo CalE6, a methionine gamma lyase from Micromonospora echinospora
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 145.204, 145.395, 346.119 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.300 - 1.850 |
| Rwork | 0.209 |
| R-free | 0.23160 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.300 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 308727 |
| <I/σ(I)> | 10.3 |
| Completeness [%] | 99.8 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 0.2 M lithium sulfate, 0.1 M Bis-Tris, 25% PEG3350 |
