4U27

Crystal structure of the E. coli ribosome bound to flopristin and linopristin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	211.970, 434.650, 623.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.364 - 2.800
R-factor	0.2155
Rwork	0.215
R-free	0.26040
RMSD bond length	0.005
RMSD bond angle	0.902
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		2.900
High resolution limit [Å]	2.800	70.000	2.800
Rmerge	0.130		0.857
Rmeas	0.155		1.076
Total number of observations	3136207
Number of reflections	1245176		120369
<I/σ(I)>	5.38		0.56
Completeness [%]	89.2		86.8
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD
1	MICROBATCH	6.5	291	PEG8k, MPD