4U22

GluA2flip sLBD complexed with FW and (R,R)-2b crystal form D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	64.170, 91.740, 48.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.286 - 1.441
R-factor	0.1894
Rwork	0.188
R-free	0.21660
RMSD bond length	0.010
RMSD bond angle	1.232
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.290
High resolution limit [Å]	1.440
Number of reflections	51673
<I/σ(I)>	19.3
Completeness [%]	99.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Na acetate, pH 4.5, 20% PEG3K