4U1X

Full length GluA2-kainate-(R,R)-2b complex crystal form B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	96.520, 160.730, 338.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.787 - 3.301
R-factor	0.2448
Rwork	0.242
R-free	0.28900
RMSD bond length	0.003
RMSD bond angle	0.819
Data reduction software	XDS
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	73.380
High resolution limit [Å]	3.200
Number of reflections	80397
<I/σ(I)>	5.4
Completeness [%]	91.6
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MES, pH 6.2, 5.25% PEG6K, 3% TMAO, 0.006 M Taurine, 20 mM MnCl2