4U1W

Full length GluA2-kainate-(R,R)-2b complex crystal form A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	104.500, 151.110, 332.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.910 - 3.250
R-factor	0.2725
Rwork	0.270
R-free	0.31610
RMSD bond length	0.004
RMSD bond angle	0.849
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	83.210
High resolution limit [Å]	3.230
Number of reflections	83881
<I/σ(I)>	11.1
Completeness [%]	98.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MES, pH 6.5, 6%PEG6K, 3% TMAO, 0.006M Taurine