4U1W
Full length GluA2-kainate-(R,R)-2b complex crystal form A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.500, 151.110, 332.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.910 - 3.250 |
R-factor | 0.2725 |
Rwork | 0.270 |
R-free | 0.31610 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.849 |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 83.210 |
High resolution limit [Å] | 3.230 |
Number of reflections | 83881 |
<I/σ(I)> | 11.1 |
Completeness [%] | 98.4 |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M MES, pH 6.5, 6%PEG6K, 3% TMAO, 0.006M Taurine |