4U1C

Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-08-30
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	155.550, 155.550, 91.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.903 - 3.500
R-factor	0.2636
Rwork	0.260
R-free	0.29280
RMSD bond length	0.006
RMSD bond angle	1.027
Refinement software	PHENIX ((phenix.refine: dev_1539))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.610
High resolution limit [Å]	3.500	3.500
Number of reflections	16368
<I/σ(I)>	27	2.3
Completeness [%]	99.9	99.8
Redundancy	17.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	PEG 6000, KSCN, Bis-Tris-propane