4U0Z
Eukaryotic Fic Domain containing protein with bound APCPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.100, 83.754, 130.023 |
| Unit cell angles | 89.92, 89.57, 89.43 |
Refinement procedure
| Resolution | 43.340 - 2.950 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cuc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.496 | |
| Number of reflections | 65073 | |
| <I/σ(I)> | 4.4 | 1.7 |
| Completeness [%] | 94.7 | |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 25% PEG 3350, 200mM Na K Tartrate, 100mM Bis-Tris Propane 7.5 |
