4U0T

Crystal structure of ADC-7 beta-lactamase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-28
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 1
Unit cell lengths	80.995, 88.571, 105.880
Unit cell angles	67.29, 89.84, 89.40

Refinement procedure

Resolution	40.000 - 1.730
R-factor	0.19616
Rwork	0.194
R-free	0.23923
RMSD bond length	0.020
RMSD bond angle	2.032
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.730
Number of reflections	273490
<I/σ(I)>	8.4
Completeness [%]	97.3
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	PEG 1500, succinate, phosphate, glycine