4U0S
Structure of Eukaryotic fic domain containing protein with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-09 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.040, 76.010, 92.030 |
Unit cell angles | 90.00, 107.56, 90.00 |
Refinement procedure
Resolution | 31.860 - 2.490 |
R-factor | 0.2093 |
Rwork | 0.207 |
R-free | 0.24460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cuc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.860 |
High resolution limit [Å] | 2.540 |
Number of reflections | 32508 |
<I/σ(I)> | 6.5 |
Completeness [%] | 98.7 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 20% peg 3350, 200mM Na K tartrate, 100mM Bis-Tris-Propane 7.5 |