4U0R

Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to monoclonal antibody 9AD4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	39.790, 86.660, 324.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.830 - 2.300
R-factor	0.2229
Rwork	0.221
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qq9 3hr5
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.860	2.400
High resolution limit [Å]	2.300	2.300
Rmerge		0.375
Number of reflections	49434
<I/σ(I)>	9.8	2
Completeness [%]	96.2	83.3
Redundancy	5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	24% PEG 1500, 20% glycerol