4U0Q

Plasmodium falciparum reticulocyte-binding protein homologue 5 (PfRH5) bound to basigin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	75.280, 109.360, 151.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.400 - 3.100
R-factor	0.2186
Rwork	0.217
R-free	0.24450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u0r 3b5h
RMSD bond length	0.008
RMSD bond angle	1.060
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.460	3.270
High resolution limit [Å]	3.100	3.100
Rmerge		0.297
Number of reflections	23402
<I/σ(I)>	9.4	2.7
Completeness [%]	99.8	99.8
Redundancy	6.1	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M lithium sulphate, 20% PEG 4000, 0.1M MES pH 6.0