4U0N

Structure of the Vibrio cholerae di-nucleotide cyclase (DncV) deletion mutant D-loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.968
Spacegroup name	P 1 21 1
Unit cell lengths	73.605, 49.253, 122.233
Unit cell angles	90.00, 98.66, 90.00

Refinement procedure

Resolution	37.730 - 2.102
R-factor	0.2198
Rwork	0.217
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u0l
RMSD bond length	0.006
RMSD bond angle	0.881
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	120.000
High resolution limit [Å]	2.100
Number of reflections	50515
<I/σ(I)>	21.1
Completeness [%]	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	16% w/v PET 3350, 0.2M MgCl2, 100mM Tris-HCl