4U0B
Hexamer HIV-1 CA in complex with CPSF6 peptide, P212121 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96860 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.130, 135.890, 208.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.600 - 2.800 |
| R-factor | 0.2311 |
| Rwork | 0.230 |
| R-free | 0.26190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u0a |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.739 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.069 | 0.588 |
| Number of reflections | 89373 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 95.1 | 97.6 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 20% v/v PEG 300, 10% v/v glycerol, 5% w/v PEG 8K, 0.1 M TRIS |
