4TZZ

Co-crystals of the Ternary Complex Containing a T-box Stem I RNA, its Cognate tRNAGly, and B. subtilis YbxF protein, treated by removing lithium sulfate and increasing PEG3350 concentration from 20% to 45% post crystallization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	70.641, 260.733, 70.698
Unit cell angles	90.00, 92.80, 90.00

Refinement procedure

Resolution	70.610 - 3.640
R-factor	0.22
Rwork	0.217
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lck
RMSD bond length	0.002
RMSD bond angle	0.493
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.610	3.770
High resolution limit [Å]	3.640	3.640
Rmerge	0.074	0.461
Number of reflections	24059
<I/σ(I)>	16.1	2.9
Completeness [%]	84.5	85.7
Redundancy	5.3	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM Bis-Tris (HCl) pH 6.5, 300 mM Li2SO4, and 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K