4TZZ
Co-crystals of the Ternary Complex Containing a T-box Stem I RNA, its Cognate tRNAGly, and B. subtilis YbxF protein, treated by removing lithium sulfate and increasing PEG3350 concentration from 20% to 45% post crystallization
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.641, 260.733, 70.698 |
Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
Resolution | 70.610 - 3.640 |
R-factor | 0.22 |
Rwork | 0.217 |
R-free | 0.26930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lck |
RMSD bond length | 0.002 |
RMSD bond angle | 0.493 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.610 | 3.770 |
High resolution limit [Å] | 3.640 | 3.640 |
Rmerge | 0.074 | 0.461 |
Number of reflections | 24059 | |
<I/σ(I)> | 16.1 | 2.9 |
Completeness [%] | 84.5 | 85.7 |
Redundancy | 5.3 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM Bis-Tris (HCl) pH 6.5, 300 mM Li2SO4, and 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |