4TXY
Crystal structure of Vibrio cholerae DncV cyclic AMP-GMP synthase, a prokaryotic cGAS homolog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2014-05-12 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.167, 59.116, 102.539 |
Unit cell angles | 90.00, 95.48, 90.00 |
Refinement procedure
Resolution | 45.120 - 3.000 |
R-factor | 0.2259 |
Rwork | 0.225 |
R-free | 0.25040 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.805 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.120 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 16701 | |
<I/σ(I)> | 6.4 | 1.3 |
Completeness [%] | 98.0 | 98.6 |
Redundancy | 3.5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 30 mM HEPES-KOH pH 7.5, 200-300 mM Mg(OAc)2 and 20-22% PEG-3350 |