4TXR
Crystal structure of LIP5 N-terminal domain complexed with CHMP1B MIM and CHMP5 MIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 193.15 |
| Detector technology | CCD |
| Collection date | 2013-04-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.99983 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.197, 60.567, 52.048 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 28.668 - 1.000 |
| R-factor | 0.1642 |
| Rwork | 0.164 |
| R-free | 0.17980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4txq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.680 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.050 | 0.270 |
| Number of reflections | 99846 | |
| <I/σ(I)> | 50.6 | 3.5 |
| Completeness [%] | 87.4 | 46.1 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277.15 | 19% PEG 4000, 0.025 M sodium acetate |
