4TX9
Crystal structure of HisAp from Streptomyces sviceus with degraded ProFAR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97927 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.546, 72.546, 143.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.840 - 1.600 |
R-factor | 0.1529 |
Rwork | 0.152 |
R-free | 0.18050 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.621 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: dev_1613)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.078 | 1.000 |
Number of reflections | 51524 | |
<I/σ(I)> | 27.8 | 1.97 |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 7.8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.2 M NaCl, 0.1 M imidazole/HCl, 0.4 M Na2HPO4/1.6 M K2HPO4, 10mg/ml protein, 2 mM ProFAR |