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4TWK

Crystal structure of human two pore domain potassium ion channel TREK1 (K2P2.1)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I24
Synchrotron siteDiamond
BeamlineI24
Temperature [K]100
Detector technologyPIXEL
Collection date2013-12-07
DetectorPSI PILATUS 6M
Wavelength(s)0.9686
Spacegroup nameP 21 21 21
Unit cell lengths74.470, 105.975, 128.270
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution33.940 - 2.600
R-factor0.2322
Rwork0.231
R-free0.25800
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4bw5
RMSD bond length0.009
RMSD bond angle1.000
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareBUSTER (2.11.4)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]39.6502.670
High resolution limit [Å]2.6002.600
Rmerge0.0731.836
Number of reflections31773
<I/σ(I)>15.51.1
Completeness [%]99.693.1
Redundancy7.36.3
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP2770.25M magnesium formate, 0.1M Sodium Cacodylate pH 6.5, 17% PEG3000, 5% PEG400

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