4TWK
Crystal structure of human two pore domain potassium ion channel TREK1 (K2P2.1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-07 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.470, 105.975, 128.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.940 - 2.600 |
R-factor | 0.2322 |
Rwork | 0.231 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bw5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.000 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.650 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.073 | 1.836 |
Number of reflections | 31773 | |
<I/σ(I)> | 15.5 | 1.1 |
Completeness [%] | 99.6 | 93.1 |
Redundancy | 7.3 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.25M magnesium formate, 0.1M Sodium Cacodylate pH 6.5, 17% PEG3000, 5% PEG400 |