4TVO

Structure of Malate Dehydrogenase from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	52.606, 79.307, 155.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.760 - 1.500
R-factor	0.1673
Rwork	0.166
R-free	0.19274
RMSD bond length	0.022
RMSD bond angle	2.066
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall
Low resolution limit [Å]	77.760
High resolution limit [Å]	1.500
Number of reflections	103872
<I/σ(I)>	13.57
Completeness [%]	99.4
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	Malate dehydrogenase crystals grew in 0.2M Lithium Sulfate monohydrate, 0.1M Tris-HCl pH=8.5, 30% w/v PEG 4000 in the 50/50 protein/reservoir-ratio drop of 1 microlitre..