4TV8
Tubulin-Maytansine complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-05 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.860, 155.570, 180.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.423 - 2.103 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i4t |
RMSD bond length | 0.002 |
RMSD bond angle | 0.664 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.500 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.139 | |
Number of reflections | 169427 | |
<I/σ(I)> | 14.2 | 0.6 |
Completeness [%] | 99.6 | 94.5 |
Redundancy | 13.4 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 6% PEG, 16% glycerol, 30 mM magnesium chloride, 30 mM calcium chloride, 0.1M MES/0.1M imidazole |