4TUF

Catalytic domain of the major endoglucanase from Xanthomonas campestris pv. campestris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-24
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	174.660, 141.531, 108.008
Unit cell angles	90.00, 110.49, 90.00

Refinement procedure

Resolution	43.720 - 2.700
R-factor	0.167
Rwork	0.165
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zum
RMSD bond length	0.004
RMSD bond angle	0.811
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	107.039	2.850
High resolution limit [Å]	2.700	2.700
Rmerge		0.619
Number of reflections	67461
<I/σ(I)>	11.5	1.3
Completeness [%]	100.0	100
Redundancy	3.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M Tris HCl pH 8.5, 2.0 M ammonium dihydrogen phosphate.