4TU3

Crystal structure of yeast Sac1/Vps74 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	H 3
Unit cell lengths	159.338, 159.338, 143.389
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.542 - 3.187
R-factor	0.2289
Rwork	0.226
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zih 3lwt
RMSD bond length	0.002
RMSD bond angle	0.453
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.542
High resolution limit [Å]	3.187
Number of reflections	22586
<I/σ(I)>	18.8
Completeness [%]	100.0
Redundancy	9.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	200mM HEPES at pH 7.0, 35% (v/v) MPD, 0.3M NDSB-195