4TTS

Crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase (Y200A) complex with 10-formyl-5,8-dideazafolate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	110
Detector technology	CCD
Collection date	2012-04-11
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	103.636, 54.378, 61.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.263 - 2.000
R-factor	0.2035
Rwork	0.201
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ts4
RMSD bond length	0.008
RMSD bond angle	1.268
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Number of reflections	23485
<I/σ(I)>	12.6
Completeness [%]	97.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	Bis-Tris (0.1~0.2 M, pH 5.5) and PEG3350 (25~29%, w/v)