4TTG

Beta-galactosidase (E. coli) in the presence of potassium chloride.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-11-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	149.290, 168.100, 200.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.810 - 1.600
R-factor	0.1602
Rwork	0.160
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dp0
RMSD bond length	0.020
RMSD bond angle	1.963
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.500	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.075	0.232
Number of reflections	604015
<I/σ(I)>	9.5	3.7
Completeness [%]	92.9	87.5
Redundancy	2.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	100 mM Bis-Tris 100 mM NaCl 200 mM MgCl2 9-12 % PEG 8000 Microseeded Serial soaked in buffer with 100 mM KCl, yielding 100 mM KCl and 16 nM NaCl Cryoprotected by serial soaks over several hours in 5% increments to 30%/70% (v/v) DMSO/above KCl buffer Cooled in the nitrogen stream at 100 K