4TSY
Crystal structure of FraC with lipids
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 151.337, 199.879, 120.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.650 - 3.140 |
R-factor | 0.19967 |
Rwork | 0.199 |
R-free | 0.22123 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vwi |
RMSD bond length | 0.013 |
RMSD bond angle | 1.698 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.650 | 3.320 |
High resolution limit [Å] | 3.140 | 3.140 |
Rmerge | 0.150 | 0.410 |
Number of reflections | 30154 | |
<I/σ(I)> | 9.9 | 3.4 |
Completeness [%] | 94.6 | 83.5 |
Redundancy | 7.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | Jeffamine M600, 1,2,3-heptanetriol, monoolein, ammonium sulfate, HEPES |