4TS2
Crystal structure of the Spinach RNA aptamer in complex with DFHBI, magnesium ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.877, 49.399, 188.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.884 |
R-factor | 0.2154 |
Rwork | 0.213 |
R-free | 0.25660 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.732 |
Data scaling software | SCALA |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.880 |
Number of reflections | 8415 |
<I/σ(I)> | 23.9 |
Completeness [%] | 99.6 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG 3350, 0.5M NaCl, 0.1M succinate-K pH 7.0, 10% sucrose |