4TR1
Crystal structure of GSH-bound cGrx2/C15S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.065, 29.371, 64.836 |
Unit cell angles | 90.00, 118.17, 90.00 |
Refinement procedure
Resolution | 32.356 - 1.582 |
R-factor | 0.179 |
Rwork | 0.177 |
R-free | 0.20430 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.449 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.580 |
Number of reflections | 27954 |
<I/σ(I)> | 39.5 |
Completeness [%] | 97.8 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | 0.1 M sodium acetate, pH 5.2, 33% PEG 4000 (w/v), and 0.2 M ammonium acetate. |