4TQO
The crystal structure of methanol dehydrogenase from Methylococcus capsulatus (Bath)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 128.490, 210.290, 231.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 105.150 - 2.570 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ad7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.933 |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.150 | 2.640 |
High resolution limit [Å] | 2.570 | 2.570 |
Number of reflections | 199109 | |
<I/σ(I)> | 10.5 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.6 | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M NaHEPES pH 7.5, 30 % PEG4000 |