4TQO

The crystal structure of methanol dehydrogenase from Methylococcus capsulatus (Bath)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	128.490, 210.290, 231.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.150 - 2.570
R-factor	0.16
Rwork	0.158
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ad7
RMSD bond length	0.005
RMSD bond angle	0.933
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.150	2.640
High resolution limit [Å]	2.570	2.570
Number of reflections	199109
<I/σ(I)>	10.5	2.6
Completeness [%]	100.0	100
Redundancy	7.6	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M NaHEPES pH 7.5, 30 % PEG4000