4TQF
Crystal Structure of the C-terminal domain of IFRS bound with 2-(5-bromothienyl)-L-Ala and ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9202 |
Spacegroup name | P 64 |
Unit cell lengths | 105.110, 105.110, 70.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.204 - 2.714 |
R-factor | 0.1726 |
Rwork | 0.168 |
R-free | 0.21120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zim |
RMSD bond length | 0.004 |
RMSD bond angle | 0.926 |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.204 |
High resolution limit [Å] | 2.710 |
Number of reflections | 12071 |
<I/σ(I)> | 39.6 |
Completeness [%] | 99.8 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | Hepes, MgCl2, PEG3350 |