4TQC

The co-complex structure of the translation initiation factor eIF4E with the inhibitor 4EGI-1 reveals an allosteric mechanism for dissociating eIF4G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	CCD
Collection date	2012-05-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 1 21 1
Unit cell lengths	40.210, 73.640, 66.980
Unit cell angles	90.00, 74.92, 90.00

Refinement procedure

Resolution	19.500 - 1.800
R-factor	0.1625
Rwork	0.161
R-free	0.19410
RMSD bond length	0.004
RMSD bond angle	0.790
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.550
High resolution limit [Å]	1.800
Number of reflections	32707
<I/σ(I)>	24.7
Completeness [%]	93.8
Redundancy	3.27

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	PEG 4000 12-20%, 100mM MES pH 8.5, 10% IPN