4TPR
Structure of Tau5 antibody Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.374, 50.408, 75.169 |
| Unit cell angles | 90.00, 115.35, 90.00 |
Refinement procedure
| Resolution | 67.930 - 1.600 |
| R-factor | 0.14502 |
| Rwork | 0.143 |
| R-free | 0.19109 |
| Starting model (for MR) | 12e8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.930 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.156 | 1.600 |
| Number of reflections | 54464 | |
| <I/σ(I)> | 10.64 | 1.22 |
| Completeness [%] | 93.9 | 64.3 |
| Redundancy | 7.236 | 6.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.2 M NaCl, 0.1 M Bis-Tris, 25 % w/v PEG 3350 |
