4TNZ
Structure basis of cellular dNTP regulation, SAMHD1-GTP-dATP-dTTP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.126, 141.749, 98.474 |
| Unit cell angles | 90.00, 116.29, 90.00 |
Refinement procedure
| Resolution | 88.290 - 2.380 |
| R-factor | 0.2132 |
| Rwork | 0.212 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bzb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.380 | 6.510 | 2.400 |
| Rmerge | 0.149 | 0.096 | |
| Total number of observations | 417301 | ||
| Number of reflections | 83563 | ||
| <I/σ(I)> | 7.4 | 0.9 | |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 5 | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 298 | SPG buffer, PEG 1500 |
