4TNW
C. elegans glutamate-gated chloride channel (GluCl) in complex with Fab and POPC in a lipid-modulated conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 453.750, 192.870, 196.140 |
Unit cell angles | 90.00, 92.27, 90.00 |
Refinement procedure
Resolution | 58.700 - 3.200 |
R-factor | 0.2281 |
Rwork | 0.227 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rhw |
RMSD bond length | 0.003 |
RMSD bond angle | 0.739 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.700 | 3.240 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.133 | 0.638 |
Number of reflections | 256166 | |
<I/σ(I)> | 7.26 | 1.43 |
Completeness [%] | 92.2 | 83.5 |
Redundancy | 1.92 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 35-36% pentaerythritol propoxylate (5/4 PO/OH), 50 mM sodium citrate pH 5.5, 100 mM potassium chloride, Crystals were obtained after incubation of protein with lipid and detergent for 4 weeks at 4 degC |