4TNT

Structure of the human mineralocorticoid receptor in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	74.090, 115.136, 81.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.231 - 2.394
R-factor	0.2136
Rwork	0.209
R-free	0.25840
RMSD bond length	0.012
RMSD bond angle	1.243
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.084	0.074	0.438
Total number of observations	80946
Number of reflections	14459
<I/σ(I)>	17.5
Completeness [%]	90.4	99.1	60.1
Redundancy	5.6	6.6	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M sodium malonate, 12 % PEG 3350