4TNP
Structural basis of cellular dNTP regulation, SAMHD1-GTP-dCTP-cCTP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.507, 140.753, 97.490 |
Unit cell angles | 90.00, 114.79, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4bzb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.474 |
Data reduction software | HKL-2000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.125 | 0.073 | |
Total number of observations | 419174 | ||
Number of reflections | 131807 | ||
<I/σ(I)> | 8.1 | ||
Completeness [%] | 98.5 | 98.1 | 98.7 |
Redundancy | 3.2 | 3.4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7.4 | 298 | SPG buffer, PEG 1500 |