4TMW
Translation initiation factor eIF5B (517-858) from C. thermophilum, bound to GTP and Sodium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.610, 116.450, 66.240 |
| Unit cell angles | 90.00, 101.15, 90.00 |
Refinement procedure
| Resolution | 43.367 - 1.550 |
| R-factor | 0.1584 |
| Rwork | 0.157 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ncn |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.757 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (2.5.2) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 | |
| High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
| Rmerge | 0.021 | 0.505 | |
| Rmeas | 0.047 | 0.025 | 0.633 |
| Total number of observations | 405555 | ||
| Number of reflections | 118431 | 1365 | 8742 |
| <I/σ(I)> | 15.52 | 62.32 | 2.3 |
| Completeness [%] | 98.8 | 98.5 | 97.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.25 | 293 | HEPES, PEG 4000, NaOAc |
