4TLV
CARDS TOXIN, NICKED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 191.698, 107.407, 222.261 |
Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
Resolution | 38.870 - 1.900 |
R-factor | 0.188 |
Rwork | 0.186 |
R-free | 0.21500 |
Structure solution method | SIRAS |
RMSD bond length | 0.010 |
RMSD bond angle | 1.099 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((PHENIX.REFINE: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 350417 | |
<I/σ(I)> | 15.5 | 2.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 1.6 M AMMONIUM SULFATE, 0.08 M SODIUM ACETATE, 20% GLYCEROL |